milk
Contents:
Installation
Option 1: Pre-compiled binary (Recommended)
Option 2: MILK installation as a Julia module
Verify successful installation
Usage
Example usage
Input
Output
MILK heuristics
High Performance Computing mode
Tutorials
MILK tutorial: Network visualization of MILK trees PBMC (3k dataset)
Importing libraries
Loading the dataset
MILK input
MILK execution
Exporting MILK directory to PATH
Visualization of the single-cell MILK tree embedding
MILK tutorial: Quantifying dynamic cellular information with MILK trees (Paul15 dataset)
Overview
Importing libraries
Loading the dataset
Standard pre-processing
MILK input
Running MILK
Processing the MILK output
Visualizing the MILK tree in R (
ggraph
package)
Downstream analyses utilizing the MILK tree output
Weighted average depth of each cell type in the MILK tree
Calculating the number of (productive) branching events from each cell (leaf) to the root
Threshold-based quantification cell type substructure in the MILK tree topology
milk
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